Protein_Domain

Part:BBa_K1408000:Design

Designed by: Stephen A Rettie   Group: iGEM14_Washington   (2014-10-09)


Degron (unstable protein domain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Xba1 and Spe1 restriction sites were in the original sequence obtained from the Fields Lab. The submitted part was synthesized with synonymous substitutions (silent mutations) amplified with primers containing the biobrick prefix and suffix and then digested and ligated into the iGEM vector.

Source

Protein domain Saccharomyces cerevisiae MATa2 (a=alpha). This part was obtained from the Fields Lab at the University of Washington.

References

Degron from Ben Jester - Fields Lab, UW